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1996-07-05
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26KB
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605 lines
LOCUS ECOLAC 7477 bp ds-DNA BCT 15-DEC-1988
DEFINITION E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
ACCESSION J01636 J01637 K01483 K01793
KEYWORDS acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
lac repressor protein; lacA gene; lacI gene; lacY gene; lacZ gene;
lactose permease; mutagenesis; palindrome; promoter;
thiogalactoside acetyltransferase.
SOURCE Escherichia coli DNA [1],[4],[5],[8],[10]; mRNA [2],[3],[6]; clone
lambda-h80dlac DNA [7],[9]; clone puk217 [11]; pgm8 [12].
ORGANISM Escherichia coli
Prokaryota; Bacteria;
Gram-negative facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1243 to 1266)
AUTHORS Gilbert,W. and Maxam,A.
TITLE The nucleotide sequence of the lac operator
JOURNAL Proc Nat Acad Sci USA 70, 3581-3584 (1973)
STANDARD full staff_review
REFERENCE 2 (bases 1246 to 1308)
AUTHORS Maizels,N.M.
TITLE The nucleotide sequence of the lactose messenger ribonucleic acid
transcribed from the UV5 promoter mutant of Escherichia coli
JOURNAL Proc Nat Acad Sci USA 70, 3585-3589 (1973)
STANDARD full staff_review
REFERENCE 3 (bases 1146 to 1282)
AUTHORS Dickson,R.C., Abelson,J., Barnes,W.M. and Reznikoff,W.S.
TITLE Genetic regulation: The lac control region
JOURNAL Science 187, 27-35 (1975)
STANDARD full staff_review
REFERENCE 4 (bases 1227 to 1271)
AUTHORS Gilbert,W., Maxam,A. and Mirzabekov,A.
TITLE Contacts between the lac repressor and DNA revealed by methylation
JOURNAL (in) Kjeldgaard,N.C. and Maaloe,O.(eds);
Control of ribosome synthesis: 138-143;
Academic Press, New York (1976)
STANDARD full staff_review
REFERENCE 5 (bases 1242 to 1268)
AUTHORS Heyneker,H.L., Shine,J., Goodman,H.M., Boyer,H.W., Rosenberg,J.,
Dickerson,R.E., Narang,S.A., Itakura,K., Lin,S. and Riggs,A.D.
TITLE Synthetic lac operator is functional in vivo
JOURNAL Nature 263, 748-752 (1976)
STANDARD full staff_review
REFERENCE 6 (bases 51 to 264)
AUTHORS Steege,D.A.
TITLE 5'-terminal nucleotide sequence of Escherichia coli lactose
repressor mRNA: Features of translational initiation and
reinitiation sites
JOURNAL Proc Nat Acad Sci USA 74, 4163-4167 (1977)
STANDARD full staff_review
REFERENCE 7 (bases 1 to 81)
AUTHORS Calos,M.P.
TITLE DNA sequence for a low-level promoter of the lac repressor and an
"up" promoter mutation
JOURNAL Nature 274, 762-765 (1978)
STANDARD full staff_review
REFERENCE 8 (bases 49 to 1161)
AUTHORS Farabaugh,P.J.
TITLE Sequence of the lacI gene
JOURNAL Nature 274, 765-769 (1978)
STANDARD full staff_review
REFERENCE 9 (bases 4306 to 5804)
AUTHORS Buechel,D.E., Gronenborn,B. and Mueller-Hill,B.
TITLE Sequence of the lactose permease gene
JOURNAL Nature 283, 541-545 (1980)
STANDARD full staff_review
REFERENCE 10 (bases 1183 to 1291)
AUTHORS Weiher,H. and Schaller,H.
TITLE Segment-specific mutagenesis: Extensive mutagenesis of a lac
promoter/operator element
JOURNAL Proc Nat Acad Sci USA 79, 1408-1412 (1982)
STANDARD full staff_review
REFERENCE 11 (bases 1287 to 4364)
AUTHORS Kalnins,A., Otto,K., Ruether,U. and Mueller-Hill,B.
TITLE Sequence of the lacZ gene of Escherichia coli
JOURNAL EMBO J 2, 593-597 (1983)
STANDARD full staff_review
REFERENCE 12 (bases 5646 to 7477)
AUTHORS Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I.
TITLE DNA sequence of the lactose operon: The lacA gene and the
transcriptional termination region
JOURNAL Proc Nat Acad Sci USA 82, 6414-6418 (1985)
STANDARD full staff_review
REFERENCE 13 (sites; UV5 mRNA transcripts and operator mutants)
AUTHORS Gilbert,W., Maizels,N. and Maxam,A.
TITLE Sequences of controlling regions of the lactose operon
JOURNAL Cold Spring Harb Symp Quant Biol 38, 845-855 (1974)
STANDARD full staff_review
REFERENCE 14 (sites; operator mutational analysis)
AUTHORS Gilbert,W., Gralla,J., Majors,A.J. and Maxam,A.
TITLE Lactose operator sequences and the action of lac repressor
JOURNAL (in) Sund,H. and Blauer,G. (eds.);
Protein-Ligand Interactions: 193-207;
Walter de Gruyter, New York (1975)
STANDARD full staff_review
REFERENCE 15 (sites; S1 and mung bean nuclease action on operator DNA)
AUTHORS Marians,K.J. and Wu,R.
TITLE Structure of the lactose operator
JOURNAL Nature 260, 360-363 (1976)
STANDARD full staff_review
REFERENCE 16 (sites; class I, II and III promoter mutant analysis)
AUTHORS Dickson,R.C., Abelson,J., Johnson,P., Reznikoff,W.S. and
Barnes,W.M.
TITLE Nucleotide sequence changes produced by mutations in the lac
promoter of Escherichia coli
JOURNAL J Mol Biol 111, 65-75 (1977)
STANDARD full staff_review
REFERENCE 17 (sites; lacI mutant analysis)
AUTHORS Miller,J.H., Coulondre,C. and Farabaugh,P.J.
TITLE Correlation of nonsense sites in the lacI gene with specific codons
in the nucleotide sequence
JOURNAL Nature 274, 770-775 (1978)
STANDARD full staff_review
REFERENCE 18 (sites; Tn5, Tn9 and Tn10 insertion sites in lac region)
AUTHORS Miller,J.H., Calos,M.P. and Galas,D.J.
TITLE Genetic and sequencing studies of the specificity of transposition
into the lac region of E. coli
JOURNAL Cold Spring Harb Symp Quant Biol 45, 243-257 (1981)
STANDARD full staff_review
REFERENCE 19 (sites; lacI promoter mutation UJ177)
AUTHORS Calos,M.P. and Miller,J.H.
TITLE DNA sequence alteration resulting from a mutation impairing
promoter function in the lac repressor gene
JOURNAL Mol Gen Genet 178, 225-227 (1980)
STANDARD full staff_review
REFERENCE 20 (sites; palindromic dimer operator;
see separate sequence entry under synthetic sequences)
AUTHORS Betz,J.L. and Sadler,J.R.
TITLE Variants of a cloned synthetic lactose operator: I. A palindromic
dimer lactose operator derived from one strand of the cloned
40-base pair operator
JOURNAL Gene 13, 1-12 (1981)
STANDARD full staff_review
REFERENCE 21 (sites; natural operator sequence)
AUTHORS Sadler,J.R. and Tecklenburg,M.
TITLE Cloning and characterization of the natural lactose operator
JOURNAL Gene 13, 13-23 (1981)
STANDARD full staff_review
REFERENCE 22 (sites; operator mutational analysis)
AUTHORS Betz,J.L. and Sadler,J.R.
TITLE Variants of a cloned synthetic lactose operator: II.
Chloramphenicol-resistant revertants retaining a lactose opeator in
the CAT gene of plasmid pBR325
JOURNAL Gene 15, 187-200 (1981)
STANDARD full staff_review
REFERENCE 23 (sites; lacI-Q deletion)
AUTHORS Calos,M.P. and Miller,J.H.
TITLE The DNA sequence change resulting from the I-Q1 mutation, which
greatly increases promoter strength
JOURNAL Mol Gen Genet 183, 559-560 (1981)
STANDARD full staff_review
REFERENCE 24 (sites; RNA polymerase UV5 promoter interaction)
AUTHORS Chenchick,A., Beabealashvilli,R. and Mirzabekov,A.
TITLE Topography of interaction of Escherichia coli RNA polymerase
subunits with lac UV5 promoter
JOURNAL FEBS Lett 128, 46-50 (1981)
STANDARD full staff_review
REFERENCE 25 (sites; lacY mutational analysis)
AUTHORS Mieschendahl,M., Buechel,D., Bocklage,H. and Mueller-Hill,B.
TITLE Mutations in the lacY gene of Escherichia coli define functional
organization of lactose permease
JOURNAL Proc Nat Acad Sci USA 78, 7652-7656 (1981)
STANDARD full staff_review
REFERENCE 26 (sites; lacI-promoted transcription termination)
AUTHORS Horowitz,H. and Platt,T.
TITLE A termination site for lacI transcription is between the CAP site
and the lac promoter
JOURNAL J Biol Chem 257, 11740-11746 (1982)
STANDARD full staff_review
REFERENCE 27 (sites; wt and UV5 promoter sequence studies)
AUTHORS Klein,R.D. and Wells,R.D.
TITLE Effects of neighboring DNA homopolymers on the biochemical and
physical properties of the Escherichia coli lactose promoter: I.
Cloning and characterization studies
JOURNAL J Biol Chem 257, 12954-12961 (1982)
STANDARD full staff_review
REFERENCE 28 (sites; UV5 promoter mutational analysis)
AUTHORS Russell,D.R. and Bennett,G.N.
TITLE Construction and analysis of in vivo activity of E. coli promoter
hybrids and promoter mutants that alter the -35 to -10 spacing
JOURNAL Gene 20, 231-243 (1982)
STANDARD full staff_review
REFERENCE 29 (sites; perfectly symmetric operator sequence)
AUTHORS Sadler,J.R., Sasmor,H. and Betz,J.L.
TITLE A perfectly symmetric lac operator binds the lac repressor very
tightly
JOURNAL Proc Nat Acad Sci USA 80, 6785-6789 (1983)
STANDARD full staff_review
REFERENCE 30 (sites; lacI mRNA termination site)
AUTHORS Cone,K.C., Sellitti,M.A. and Steege,D.A.
TITLE Lac repressor mRNA transcription terminates in vivo in the lac
control region
JOURNAL J Biol Chem 258, 11296-11304 (1983)
STANDARD full staff_review
REFERENCE 31 (sites; distamycin and actinomycin binding to promoter)
AUTHORS Van Dyke,M.W. and Dervan,P.B.
TITLE Footprinting with MPE-Fe(II). Complementary-strand analyses of
distamycin- and actinomycin-binding sites on heterogeneous DNA
JOURNAL Cold Spring Harb Symp Quant Biol 47, 347-353 (1983)
STANDARD full staff_review
REFERENCE 32 (sites; lacI deletion studies)
AUTHORS Glickman,B.W. and Ripley,L.S.
TITLE Structural intermediates of deletion mutagenesis: A role for
palindromic DNA
JOURNAL Proc Nat Acad Sci USA 81, 512-516 (1984)
STANDARD full staff_review
REFERENCE 33 (sites; promoter mutational studies)
AUTHORS Mandecki,W., Goldman,R.A., Powell,B.S. and Caruthers,M.H.
TITLE Lac up-promoter mutants with increased homology to the consensus
promoter sequence
JOURNAL J Bacteriol 164, 1353-1355 (1985)
STANDARD full staff_review
REFERENCE 34 (sites; DNAase I studies with promoter sequence)
AUTHORS Straney,D.C. and Crothers,D.M.
TITLE Intermediates in transcription initiation from the E. coli lac UV5
promoter
JOURNAL Cell 43, 449-459 (1985)
STANDARD full staff_review
REFERENCE 35 (sites; ribosomal binding and translation initiation for lacZ)
AUTHORS Looman,A.C., de Gruyter,M., Vogelaar,A. and van Knippenberg,P.H.
TITLE Effects of heterologous ribosomal binding sites on the
transcription and translation of the lacZ gene of Escherichia coli
JOURNAL Gene 37, 145-154 (1985)
STANDARD full staff_review
REFERENCE 36 (sites; insertion sequence IS1 integration in lacZ;
see separate sequence entry for the IS1T sequence)
AUTHORS Malamy,M.H., Rahaim,P.T., Hoffman,C.S., Baghdoyan,D.,
O'Connor,M.B. and Miller,J.F.
TITLE A frameshift mutation at the junction of an IS1 insertion within
lacZ restores beta-galactosidase activity via formation of an
active lacZ-IS1 fusion protein
JOURNAL J Mol Biol 181, 551-555 (1985)
STANDARD full staff_review
REFERENCE 37 (sites; DNAase I studies with promoter)
AUTHORS Spassky,A., Kirkegaard,K. and Buc,H.
TITLE Changes in the DNA structure of the lac UV5 promoter during
formation of an open complex with Escherichia coli RNA polymerase
JOURNAL Biochemistry-USA 24, 2723-2731 (1985)
STANDARD full staff_review
COMMENT [1] first reports a 27 bp operator(sites 1240-1266) with two-fold
symmetries; the operator has also been defined to be bases
1246-1266 or bases 1239-1273 [5]. [4] explores the ability of lac
repressor protein to affect methylation of operator DNA. [5]
argues that DNA on both sides of the 21 bp operator (bases
1246-1266) affects repressor binding but that the sequences of this
DNA are probably not critical. [3] gives a larger sequence known as
the promoter-operator region for the wild-type, whereas [2] and
[10] give portions of this region for the mutant strain UV5.
Within the promoter region, bases 1162-1199 are identified as the
catabolite gene activator protein binding site (cap) and bases
1200-1245 are the RNA polymerase interaction site. [6] reports a
sequence for the 5'end of the lacI (repressor) gene and discusses
restart in mutant strains. [7] presents a sequence for the lacI
promoter region and identifies an I-Q mutation which enhances lacI
transcription approximately ten-fold. [8] gives a complete sequence
for lacI which agrees with the known lac repressor sequence. [10]
examines the promoter-operator region in the UV5 strain (lac109)
and studies 23 mutant derivatives of this sequence.
This sequence agrees with known protein sequences for the lacZ,
lacY and lacA enzymes. [9] notes that the fMet codon is not present
for lacA and suggests that the "ttg" codon (5727-5729), which
immediately precedes the mature N-terminal asparagine codon, is the
start codon. The cds for lacZ, lacY and lacA are included on a
single mRNA transcript.
FEATURES from to/span description
pept 79 1161 lac repressor protein (lacI; gtg start codon)
pept 1284 4358 beta-d-galactosidase (lacZ)
pept 4410 5663 lactose permease (lacY)
pept 5727 6338 thiogalactoside acetyltransferase (lacA; ttg
start codon)
mRNA 51 1230 lacI (repressor) mRNA; preferred in vivo 3'
end [8],[30]
mRNA 1246 > 4358 lacZ mRNA [2],[3]
mut 16 16 c in wild-type; t in 'up' promoter mutant I-Q
[7]
refnumbr 51 51 numbered 1 in [7],[8]; zero not used[7]
refnumbr 79 81 numbered codon 1 in [6]
signal 1162 1199 cap protein binding site
refnumbr 1162 1162 numbered 1 in [3]; zero not used
variant 1183 1186 ttag in wild-type; aatt in strain UV5 [10]
mut 1209 1211 gct in wild-type; gt in mutant l305 [3]
mut 1212 1212 t in wild-type; a in mutant l241 [3]
mut 1230 1230 c in wild-type; a in mutant p-r-1a [3]
variant 1237 1238 gt in wild-type; aa in strain UV5 [10]
mut 1242 1245 gtgg in wild-type; ttca in synthetic operator
[5]
refnumbr 1243 1243 sequence not numbered in [1]
signal 1246 1266 lac repressor protein binding site
refnumbr 1246 1246 numbered 1 in [2],[10]; zero not used
refnumbr 1247 1247 numbered 1 in [5]; zero used
revision 1262 1262 c in [2],[3],[4],[5],[10]; g in [1]
mut 1267 1268 tc in wild-type; tg in synthetic operator [5]
refnumbr 1281 1281 (c) numbered 1 on comp strand in [4]
variant 1282 1291 ctatgaccat in wild-type; gatccggcca in strain
UV5 [10]
refnumbr 1287 1287 numbered 1 in [11]
refnumbr 4305 4305 numbered 1 in [9]
refnumbr 5646 5646 numbered 4401 in [12]
BASE COUNT 1739 a 1991 c 2004 g 1743 t
ORIGIN HindII site [7]; 7.9 min on K12 map.
BACTERIA:ECOLAC Length: 7477 May 7, 1989 20:07 Check: 4810 ..
1 GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC
51 GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG
101 ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG
151 AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC
201 GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG
251 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC
301 GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG
351 TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA
401 AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT
451 AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC
501 TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA
551 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG
601 GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC
651 TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA
701 ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC
751 GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC
801 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA
851 CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC
901 GATACCGAAG ACAGCTCATG TTATATCCCG CCGTCAACCA CCATCAAACA
951 GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT
1001 CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG
1051 AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
1101 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
1151 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
1201 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG
1251 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGGA
1301 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA
1351 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT
1401 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA
1451 TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA
1501 GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA
1551 AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTAACCTA
1601 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT
1651 GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG
1701 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA
1751 CGGGCGCTGG GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG
1801 ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG
1851 CTGCGTTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT
1901 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA
1951 TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT
2001 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG
2051 GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG
2101 CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC
2151 GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT
2201 CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC
2251 TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA
2301 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA
2351 CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA
2401 TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC
2451 TGTTCGCATT ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA
2501 CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC GGCATGGTGC
2551 CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA
2601 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT
2651 CTGGTCGCTG GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT
2701 ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA GTATGAAGGC
2751 GGCGGAGCCG ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG
2801 CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG TCCATCAAAA
2851 AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC
2901 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC
2951 GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG
3001 ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC
3051 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG
3101 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG GAAGCAAAAC
3151 ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG
3201 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT
3251 GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG
3301 TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG
3351 GAGAGCGCCG GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC
3401 GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG CAGTGGCGTC
3451 TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG
3501 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG
3551 TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG
3601 ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG
3651 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC
3701 CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC GAAGCAGCGT
3751 TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC
3801 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC
3851 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG
3901 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG
3951 GCGCAGGTAG CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA
4001 CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG GATCTGCCAT
4051 TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC
4101 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT
4151 CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC
4201 ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT
4251 TTCCATATGG GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC
4301 GGAATTCCAG CTGAGCGCCG GTCGCTACCA TTACCAGTTG GTCTGGTGTC
4351 AAAAATAATA ATAACCGGGC AGGCCATGTC TGCCCGTATT TCGCGTAAGG
4401 AAATCCATTA TGTACTATTT AAAAAACACA AACTTTTGGA TGTTCGGTTT
4451 ATTCTTTTTC TTTTACTTTT TTATCATGGG AGCCTACTTC CCGTTTTTCC
4501 CGATTTGGCT ACATGACATC AACCATATCA GCAAAAGTGA TACGGGTATT
4551 ATTTTTGCCG CTATTTCTCT GTTCTCGCTA TTATTCCAAC CGCTGTTTGG
4601 TCTGCTTTCT GACAAACTCG GGCTGCGCAA ATACCTGCTG TGGATTATTA
4651 CCGGCATGTT AGTGATGTTT GCGCCGTTCT TTATTTTTAT CTTCGGGCCA
4701 CTGTTACAAT ACAACATTTT AGTAGGATCG ATTGTTGGTG GTATTTATCT
4751 AGGCTTTTGT TTTAACGCCG GTGCGCCAGC AGTAGAGGCA TTTATTGAGA
4801 AAGTCAGCCG TCGCAGTAAT TTCGAATTTG GTCGCGCGCG GATGTTTGGC
4851 TGTGTTGGCT GGGCGCTGTG TGCCTCGATT GTCGGCATCA TGTTCACCAT
4901 CAATAATCAG TTTGTTTTCT GGCTGGGCTC TGGCTGTGCA CTCATCCTCG
4951 CCGTTTTACT CTTTTTCGCC AAAACGGATG CGCCCTCTTC TGCCACGGTT
5001 GCCAATGCGG TAGGTGCCAA CCATTCGGCA TTTAGCCTTA AGCTGGCACT
5051 GGAACTGTTC AGACAGCCAA AACTGTGGTT TTTGTCACTG TATGTTATTG
5101 GCGTTTCCTG CACCTACGAT GTTTTTGACC AACAGTTTGC TAATTTCTTT
5151 ACTTCGTTCT TTGCTACCGG TGAACAGGGT ACGCGGGTAT TTGGCTACGT
5201 AACGACAATG GGCGAATTAC TTAACGCCTC GATTATGTTC TTTGCGCCAC
5251 TGATCATTAA TCGCATCGGT GGGAAAAACG CCCTGCTGCT GGCTGGCACT
5301 ATTATGTCTG TACGTATTAT TGGCTCATCG TTCGCCACCT CAGCGCTGGA
5351 AGTGGTTATT CTGAAAACGC TGCATATGTT TGAAGTACCG TTCCTGCTGG
5401 TGGGCTGCTT TAAATATATT ACCAGCCAGT TTGAAGTGCG TTTTTCAGCG
5451 ACGATTTATC TGGTCTGTTT CTGCTTCTTT AAGCAACTGG CGATGATTTT
5501 TATGTCTGTA CTGGCGGGCA ATATGTATGA AAGCATCGGT TTCCAGGGCG
5551 CTTATCTGGT GCTGGGTCTG GTGGCGCTGG GCTTCACCTT AATTTCCGTG
5601 TTCACGCTTA GCGGCCCCGG CCCGCTTTCC CTGCTGCGTC GTCAGGTGAA
5651 TGAAGTCGCT TAAGCAATCA ATGTCGGATG CGGCGCGACG CTTATCCGAC
5701 CAACATATCA TAACGGAGTG ATCGCATTGA ACATGCCAAT GACCGAAAGA
5751 ATAAGAGCAG GCAAGCTATT TACCGATATG TGCGAAGGCT TACCGGAAAA
5801 AAGACTTCGT GGGAAAACGT TAATGTATGA GTTTAATCAC TCGCATCCAT
5851 CAGAAGTTGA AAAAAGAGAA AGCCTGATTA AAGAAATGTT TGCCACGGTA
5901 GGGGAAAACG CCTGGGTAGA ACCGCCTGTC TATTTCTCTT ACGGTTCCAA
5951 CATCCATATA GGCCGCAATT TTTATGCAAA TTTCAATTTA ACCATTGTCG
6001 ATGACTACAC GGTAACAATC GGTGATAACG TACTGATTGC ACCCAACGTT
6051 ACTCTTTCCG TTACGGGACA CCCTGTACAC CATGAATTGA GAAAAAACGG
6101 CGAGATGTAC TCTTTTCCGA TAACGATTGG CAATAACGTC TGGATCGGAA
6151 GTCATGTGGT TATTAATCCA GGCGTCACCA TCGGGGATAA TTCTGTTATT
6201 GGCGCGGGTA GTATCGTCAC AAAAGACATT CCACCAAACG TCGTGGCGGC
6251 TGGCGTTCCT TGTCGGGTTA TTCGCGAAAT AAACGACCGG GATAAGCACT
6301 ATTATTTCAA AGATTATAAA GTTGAATCGT CAGTTTAAAT TATAAAAATT
6351 GCCTGATACG CTGCGCTTAT CAGGCCTACA AGTTCAGCGA TCTACATTAG
6401 CCGCATCCGG CATGAACAAA GCGCAGGAAC AAGCGTCGCA TCATGCCTCT
6451 TTGACCCACA GCTGCGGAAA ACGTACTGGT GCAAAACGCA GGGTTATGAT
6501 CATCAGCCCA ACGACGCACA GCGCATGAAA TGCCCAGTCC ATCAGGTAAT
6551 TGCCGCTGAT ACTACGCAGC ACGCCAGAAA ACCACGGGGC AAGCCCGGCG
6601 ATGATAAAAC CGATTCCCTG CATAAACGCC ACCAGCTTGC CAGCAATAGC
6651 CGGTTGCACA GAGTGATCGA GCGCCAGCAG CAAACAGAGC GGAAACGCGC
6701 CGCCCAGACC TAACCCACAC ACCATCGCCC ACAATACCGG CAATTGCATC
6751 GGCAGCCAGA TAAAGCCGCA GAACCCCACC AGTTGTAACA CCAGCGCCAG
6801 CATTAACAGT TTGCGCCGAT CCTGATGGCG AGCCATAGCA GGCATCAGCA
6851 AAGCTCCTGC GGCTTGCCCA AGCGTCATCA ATGCCAGTAA GGAACCGCTG
6901 TACTGCGCGC TGGCACCAAT CTCAATATAG AAAGCGGGTA ACCAGGCAAT
6951 CAGGCTGGCG TAACCGCCGT TAATCAGACC GAAGTAAACA CCCAGCGTCC
7001 ACGCGCGGGG AGTGAATACC ACGCGAACCG GAGTGGTTGT TGTCTTGTGG
7051 GAAGAGGCGA CCTCGCGGGC GCTTTGCCAC CACCAGGCAA AGAGCGCAAC
7101 AACGGCAGGC AGCGCCACCA GGCGAGTGTT TGATACCAGG TTTCGCTATG
7151 TTGAACTAAC CAGGGCGTTA TGGCGGCACC AAGCCCACCG CCGCCCATCA
7201 GAGCCGCGGA CCACAGCCCC ATCACCAGTG GCGTGCGCTG CTGAAACCGC
7251 CGTTTAATCA CCGAAGCATC ACCGCCTGAA TGATGCCGAT CCCCACCCCA
7301 CCAAGCAGTG CGCTGCTAAG CAGCAGCGCA CTTTGCGGGT AAAGCTCACG
7351 CATCAATGCA CCGACGGCAA TCAGCAACAG ACTGATGGCG ACACTGCGAC
7401 GTTCGCTGAC ATGCTGATGA AGCCAGCTTC CGGCCAGCGC CAGCCCGCCC
7451 ATGGTAACCA CCGGCAGAGC GGTCGAC